SUBJECT CLASSIFICATION (Example: RNOMICS)

Preeclampsia is a common pregnancy specific syndrome that is more often associated with trophoblast dysfunction during the first trimester of pregnancy. Trophoblast plays a central role in determining fetal growth and the development of pregnancy complications as wells as in programming of cardiovascular and metabolic disease in the developing fetus. Infection specific inflammation is a primary mediator of trophoblast complication. The role of U. urealyticum in altering Preeclamptic trophoblast complications are largely overlooked under such condition. Heat shock protein 70 (HSP70) is a molecular chaperone playing significant role in control of preeclamptic progression and protection of the developing fetus. c-jun N terminal Kinase (JNK) is a signaling molecule having potential role in apoptosis regulation. During preeclampsia and infection there is an altered balance between proliferation and apoptosis of villous trophoblast. There was a significant decrease in the trophoblast, endothelial cell viability in U. urealyticum infected preeclamptic (p<0.001), uninfected preeclamptic (p<0.05) cells when compared to normotensive trophoblast and endothelial cell. The changes in the expression of apoptosis regulating signal JNK in response to cytoprotective HSP70 level in the 3 study groups revealed the anti-apoptotic role of HSP70 in restoring trophoblast turnover during conditions of preeclampsia and U. urealyticum infection. The study result emphasizes the importance of HSP70 analysis under conditions of preeclamptic U. urealyticum infection. _____________________________________________________